The enterotoxin D plasmid ofStaphylococcus aureusencodes a second enterotoxin determinant (sej)

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The enterotoxin D plasmid of Staphylococcus aureus encodes a second enterotoxin determinant (sej).

Staphylococcus aureus enterotoxin D is one of the serotypes most commonly associated with food poisoning. Further characterization of the enterotoxin D-encoding plasmid revealed the presence of an open reading frame which encodes a previously unidentified enterotoxin, designated staphylococcal enterotoxin J (SEJ). SEJ is a protein of 269 amino acid residues which has substantial sequence simila...

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Culture filtrates of Shigellaflexneri 2a strain M4243 grown in iron-depleted medium, caused significant fluid accumulation in rabbit ileal loops. Also, when tested in Ussing chambers, a greater rise in potential difference and short circuit current was seen with such filtrates compared with the medium control. Analogous filtrates from two M4243 derivatives lacking the 140-MD invasiveness plasmi...

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Chromatofocusing in the purification of staphylococcal enterotoxin D.

A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enteroto...

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Detection of Staphylococcus Aureus Enterotoxin Genes A-E

Abstract Background and Objective: The main cause of spreading staphylococcal infections among patients is the healthy carriers working in hospitals. With the secretion of different sorts of toxins such as entrotoxin, this bacteria can provide the conditions for attacking on the host. The main objective of this study is identification of the characteristics and differences in the Staphylococc...

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The staphylococcal enterotoxin (SE) family

Staphylococcus aureus plays an important role in numerous human cases of food poisoning, soft tissue, and bone infections, as well as potentially lethal toxic shock. This common bacterium synthesizes various virulence factors that include staphylococcal enterotoxins (SEs). These protein toxins bind directly to major histocompatibility complex class II on antigen-presenting cells and specific Vβ...

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ژورنال

عنوان ژورنال: FEMS Microbiology Letters

سال: 1998

ISSN: 0378-1097,1574-6968

DOI: 10.1111/j.1574-6968.1998.tb13278.x